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The Panama Canal, Pearl Islands and Darién Jungle Small Ship Cruise
Embark on our only Central American expedition that travels solely through Panama, connecting you more deeply with the region’s wildlife, culture and famed modern engineering marvel. Aboard the National Geographic Quest, transit the historic Panama Canal by day and by night. Learn about the country’s indigenous and colonial history while visiting local communities and two UNESCO World Heritage sites. Encounter monkeys, iguanas, tropical birds and more as you venture into lush rainforests, a wildlife refuge and an important national park. In the surrounding turquoise waters, snorkel pristine coral reefs which shelter an array of colorful marine life.
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The Panama Canal, Pearl Islands and Darién Jungle Small Ship Cruise itinerary:
Arrive in Panama City, the capital of Panama this afternoon and transfer to Port Balboa to embark the National Geographic Quest this evening. You may opt for a culturally-immersive pre-voyage extension in Panama City and San Lorenzo, a UNESCO-designated World Heritage Site. Later on, you'll enter the Panama Canal at the Gatun Locks.
Arrival Time: Arrive by 12:00 p.m.
Arrival City: Panama City, Panama
This morning, arrive along the coast of the Darién Province. Punta Patiño Nature Reserve is Panama’s largest private nature reserve and boasts a rich tapestry of habitats that foster biodiversity. Today you will reach the local beaches by Zodiac. Overhead, search for a variety of birds including mangrove hawks, laughing falcons and roseate spoonbills, or, by land, look for crab-eating raccoons and crocodiles. Additional opportunities may be available to walk the beach, kayak in the shallow bay or continue to explore by Zodiac.
Today, choose hikes and Zodiac cruises at the Smithsonian Tropical Research Institute facility on Barro Colorado Island, or take a boat ride to Gamboa, where the Chagres River meets the Panama Canal, and hike on the Panama Rainforest Discovery Center trails. This evening, continue through the complex lock system of the Canal and venture into the Gulf of Panama.
Approximately 200 islands make up the idyllic Pearl Islands. Explore several of the islands in the archipelago, including Isla del Rey and Isla Pacheca, as you learn about the history of the native populations and eventually the Spaniards and African slaves who arrived for the pearl trade. Deep rainforests, lush rivers, white sandy beaches and shallow reefs provide great opportunities for hiking, sea kayaking, stand-up paddleboarding, Zodiac river cruises, beach activities and snorkeling.
Playa Muerto village is located in Darién National Park, the largest protected area in Central America and a UNESCO World Heritage site. The park contains an exceptional array of habitats, from sandy beaches and mangroves to dense tropical forests teeming with wildlife. It is also home to the indigenous Emberá and Wounaan tribes. You’ll be greeted by village leaders from the Emberá who will share their customs, traditions and stories about their deep respect for their natural surroundings. Later on, enjoy short nature walks, go birding or choose to join a longer hike into the rainforest in search of the elusive harpy eagle, one of the world’s most powerful eagles.
This morning, explore the historically important town of Portobelo, located on the northern coast of the isthmus of Panama. Portobelo is known for its Spanish colonial military architecture, which is designated collectively as a UNESCO World Heritage site. Visit several of the fortifications as well as the old Customs House and hear the tales of Portobelo’s rich pirate history. During your visit, enjoy a Congo dance presentation, deeply rooted in the traditions of the Afro-Panamanian community, holding great historical significance.
Later on, set sail for the Panama Canal. As cargo ships tend to pass through the canal during the day, you'll most likely begin your crossing at night, when the canal is dramatically lit. By special arrangement, National Geographic Quest will anchor in Gatún Lake near the Barro Colorado Nature Monument overnight.
After breakfast, disembark in Colón and transfer to the airport in Panama City for your flight home. Ask your expedition specialist about enjoying a culturally-immersive post-voyage extension in Panama City and San Lorenzo, a UNESCO-designated World Heritage Site.
Departure Time: Depart anytime after 2:30 p.m.
Departure City: Panama City, Panama
Itineraries are subject to change.
The Panama Canal, Pearl Islands and Darién Jungle Small Ship Cruise reverse itinerary:
Itineraries are subject to change.
After breakfast, disembark in Colón and transfer to the airport in Panama City for your flight home. Ask your expedition specialist about enjoying a culturally-immersive post-voyage extension in Panama City and San Lorenzo, a UNESCO-designated World Heritage Site.
Departure Time: Depart anytime after 2:30 p.m.
Departure City: Panama City, Panama
This morning, explore the historically important town of Portobelo, located on the northern coast of the isthmus of Panama. Portobelo is known for its Spanish colonial military architecture, which is designated collectively as a UNESCO World Heritage site. Visit several of the fortifications as well as the old Customs House and hear the tales of Portobelo’s rich pirate history. During your visit, enjoy a Congo dance presentation, deeply rooted in the traditions of the Afro-Panamanian community, holding great historical significance.
Later on, set sail for the Panama Canal. As cargo ships tend to pass through the canal during the day, you'll most likely begin your crossing at night, when the canal is dramatically lit. By special arrangement, National Geographic Quest will anchor in Gatún Lake near the Barro Colorado Nature Monument overnight.
Playa Muerto village is located in Darién National Park, the largest protected area in Central America and a UNESCO World Heritage site. The park contains an exceptional array of habitats, from sandy beaches and mangroves to dense tropical forests teeming with wildlife. It is also home to the indigenous Emberá and Wounaan tribes. You’ll be greeted by village leaders from the Emberá who will share their customs, traditions and stories about their deep respect for their natural surroundings. Later on, enjoy short nature walks, go birding or choose to join a longer hike into the rainforest in search of the elusive harpy eagle, one of the world’s most powerful eagles.
Approximately 200 islands make up the idyllic Pearl Islands. Explore several of the islands in the archipelago, including Isla del Rey and Isla Pacheca, as you learn about the history of the native populations and eventually the Spaniards and African slaves who arrived for the pearl trade. Deep rainforests, lush rivers, white sandy beaches and shallow reefs provide great opportunities for hiking, sea kayaking, stand-up paddleboarding, Zodiac river cruises, beach activities and snorkeling.
Today, choose hikes and Zodiac cruises at the Smithsonian Tropical Research Institute facility on Barro Colorado Island, or take a boat ride to Gamboa, where the Chagres River meets the Panama Canal, and hike on the Panama Rainforest Discovery Center trails. This evening, continue through the complex lock system of the Canal and venture into the Gulf of Panama.
This morning, arrive along the coast of the Darién Province. Punta Patiño Nature Reserve is Panama’s largest private nature reserve and boasts a rich tapestry of habitats that foster biodiversity. Today you will reach the local beaches by Zodiac. Overhead, search for a variety of birds including mangrove hawks, laughing falcons and roseate spoonbills, or, by land, look for crab-eating raccoons and crocodiles. Additional opportunities may be available to walk the beach, kayak in the shallow bay or continue to explore by Zodiac.
Arrive in Panama City, the capital of Panama this afternoon and transfer to Port Balboa to embark the National Geographic Quest this evening. You may opt for a culturally-immersive pre-voyage extension in Panama City and San Lorenzo, a UNESCO-designated World Heritage Site. Later on, you'll enter the Panama Canal at the Gatun Locks.
Arrival Time: Arrive by 12:00 p.m.
Arrival City: Panama City, Panama
National Geographic Quest
Vessel Type: Expedition Ship
Length: 72 metres
Passenger Capacity: 100 guests (in 50 cabins)
Built: 2017
Built in 2017, National Geographic Quest is a modern expedition vessel purpose-designed for the narrow channels and shallow coves of Pan-American waters. With insights drawn from nearly four decades of exploration in Alaska, Baja California, Costa Rica and beyond, she is perfectly equipped for immersive coastal voyages. Clean-lined and contemporary in design, her expansive glass windows ensure guests are constantly connected to the surrounding landscapes, whether spotting whales in a lagoon or cruising past rainforest-covered shores.
Accommodating 100 guests in 50 comfortable cabins across four decks, Quest features twin Zodiac boarding platforms for swift disembarkation, a shallow draught for close-to-shore navigation, and wide-ranging public areas. Guests can enjoy a relaxed dining experience with sustainable, locally inspired cuisine, unwind on the sundeck, or visit the lounge, gym, or Global Gallery. Open access to the Bridge invites a deeper engagement with navigation and wildlife spotting alongside the Captain and crew. Every detail is crafted to maximise discovery, comfort, and a shared sense of adventure.









• Discover the storied Spanish architecture and fascinating pirate history of Portobelo, a UNESCO World Heritage site
• Meet the indigenous Emberá people of Darién in Playa Muerto Village and learn about their customs, traditions and deep reverence for their natural surroundings
• Sea kayak, hike, Zodiac cruise and snorkel in the tranquil Pearl Islands, a network of 200 islands known for turquoise waters, white-sand beaches and an enduring connection to the pearl trade